Document Type : Original Article
Authors
1
Clinical Tuberculosis and Epidemiology Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran
2
Diabetes Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran
3
Laser Application in Medical Sciences Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
4
Department of Lab Sciences, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
5
Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran
6
Cell Therapy and Regenerative Medicine Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran
7
Iranian Cancer Control Center (MACSA), Tehran, Iran
8
Proteomics Research Center, System Biology Institute, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Abstract
Background: X-ray radiation application in radiology is a challenging subject for patients. Understanding the molecular events triggered by X-ray radiation can provide a suitable protocol for maintaining patient health. The primary aim of this study is to explore crucial affected genes in this regard.
Materials and Methods: Extraction and validation of data from the Gene Expression Omnibus (GEO) database is the first step in identifying significantly differentially expressed genes (DEGs). Analysis of gene expression profiles of human bronchial epithelial BEAS-2B cells through a directed protein-protein interaction (PPI) network was used to identify the key targeted genes affected by X-ray radiation.
Results: Results of the analyzed directed PPI networks, based on expression, activation, inhibition, and reaction actions, and considering outdegree values, showed that IL1B, CCND1, RAC2, NDC80, and MIB1 are the crucial genes targeted by X-ray.
Conclusion: In conclusion, upregulation of IL1B, RAC2, and CCND1, which are associated with cellular damages and downregulation of NDC80 and MIB1, which protect the treated cells, were pointed out as the major molecular events in response to X-ray radiation.
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