Document Type : Original Article
Authors
1
Department of Modern Sciences & Technologies, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
2
Division of Neurocognitive Sciences, Psychiatry and Behavioral Sciences Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
3
Craniomaxillofacial Research Center, Tehran University of Medical Sciences, Tehran, Iran, Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
4
Management Sciences and Health Economic Department, School of Public Health, Health Information Management Research Center, Tehran University of Medical Sciences, Tehran Iran
5
Chronic Respiratory Diseases Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran
6
Division of Applied Medicine, Medical School, University of Aberdeen, Forester hill, Aberdeen, UK
7
Department of Biostatistics, Mycobacteriology Research Center, NRITLD, Shahid Beheshti University of Medical Sciences, Iran
8
Department of Pathology, School of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
9
Shiraz Molecular Pathology Research Cenetr, Dr Daneshbod Lab, Shiraz, Iran
10
Department of Pharmaceutical Chemistry Faculty of Islamic Azad University of Medical Sciences, Tehran, Iran
11
Chronic Respiratory Diseases Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, IranResearch Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran
Abstract
Background: Cancer is a disease caused by manifestation and abnormal gene expression. Many of the genes that inhibit cancer by the microRNAs. The aim of this study was to investigate the Expression of miR155 gene and CEA and VEGF proteins as Diagnostic Markers in Early Stages of Non-Small Cell Lung Cancer (NSCLC).
Materials and Methods: Fifty pairs of non-small lung cancer specimens of patients from healthy and tumors specimens were collected based on physical examination and diagnosis of an expert, that, in Masih Daneshvari Hospital. 50 healthy volunteers as a control group were volunteered by the physician after examination and filled out the consent form in this study. From all subjects, 6 ml of peripheral blood were taken and examined by Real-Time PCR (RT-PCR) and ELISA.
Results: The expression level of miR-155 in patients was significantly increased compared to the control group (p<0.001). VEGF protein was positive in 34 of the 50 patients and in healthy subjects, 3 persons were positive. The statistical comparison of the amount of positive biomarker was performed in two groups and it was shown that there is a statistically significant difference between these two groups (P<0.001). CEA protein was positive in 38 of the 50 patients and 5 in healthy subjects were positive. The statistical comparison of the amount of positive biomarker was performed in two groups and it was shown that there is a statistically significant difference between these two groups (P<0.001).
Conclusion: This study showed that miR155 gene and CEA and VEGF proteins are relatively good markers for the diagnosis of non-small cell lung cancer patients in Iranian population.
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