Development of a New Method for Sampling and Analysis of Biomarker of Lipid Peroxidation in Exhaled Breath


1 Faculty of Public Health, Shaheed Beheshti University of Medical Sciences and Health Services, TEHRAN-IRAN,

2 Department of Environmental Health Sciences, School of Public Health, University of California Los Angeles, Los Angeles-California, USA


Background: The measurement of breath ethane and pentane has offered noninvasive method for evaluating of lipid peroxidation in occupations exposed to free radicals and whole body radiation. However, the methods introduced up to this date have particular complexities, that makes such measurements not easily accessible for routine monitoring of high risk occupational groups. Materials and Methods: The new sampler consists of a 5-centimeter stainless steel syringe packed with Carboxen - 1000. Sampler, after heat conditioning (200 0 C for 2 hours) was utilized for adsorption of breath alkanes and isoprene, it was subsequently injected in the injection port of gas chromatography (GC) for analysis. Results: Breath pentane and isoprene were analyzed just with gas chromatograph without auxiliary cryfocusing equipment or a thermal desorber with sensitivity of ≥15 ρmol/l. Humidity of breath volume up to 320ml did not cause problems in sampling and analysis of breath alkanes. Conclusion: This study introduces a reliable and practical method for to measure breath pentane and isoprene. (Tanaffos 2002; 1(4): 51-59)