Detection of Burkholderia cepacia Complex in Patients with Cystic Fibrosis


1 Department of Microbiology, Faculty of Medical Sciences, Tarbiat Modarres University

2 Department of Microbiology, Faculty of Medical Sciences, Tarbiat Modarres University,

3 Children Medical Center, Tehran University of Medical Sciences and Health Services,

4 Department of Biotechnology, Pasteur Institute, TEHRAN-IRAN


Background: Damaging of the lung function in patients with cystic fibrosis is the most frequent cause of death in these patients. Recently, Burkholderia cepacia has been emerged as an important opportunistic pathogen in these patients; because of its increased isolation from patients with cystic fibrosis since late1970s, the capacity for spread of infection among the cystic fibrosis patient community, its role in damaging lung functions, and its innate multiantibiotic resistance. These different aspects make isolation of Burkholderia cepacia an important task in cystic fibrosis health care settings. Materials and Methods: We examined the capacity of Burkholderia cepacia selective agar (BCSA) as a medium for primary isolation of Burkholderia cepacia samples. Biochemical tests were used to confirm the identification. Results: Burkholderia cepacia strains were isolated from 6 out of 53 respiratory samples as confirmed with biochemical tests. Conclusion: Results of the present study suggest that BCSA can be used as a selective medium with high specificity for primary isolation and identification of Burkholderia cepacia complex bacteria. (Tanaffos 2004; 3(9): 47-52)