National Research Institute of Tuberculosis and Lung Disease (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran TANAFFOS (Respiration) 1735-0344 7 2(spiring) 2008 06 01 Mutations in rpoB Gene and Genotypes of Rifampin Resistant Mycobacterium Tuberculosis Isolates in Iran 11 17 241256 EN Farahnoosh Doustdar Deprtment of Microbiology, School of Medicine & Infectious and Tropical Diseases Research center, Ahwaz Jondishapour University of Medical Sciences, Ahwaz, Iran. Mycobacteriology Research Center, NRITLD, Shahid Beheshti University, M.C., Azar Dokht Khosravi Deprtment of Microbiology, School of Medicine & Infectious and Tropical Diseases Research center, Ahwaz Jondishapour University of Medical Sciences, Ahwaz, Iran. Parissa Farnia Mycobacteriology Research Center, NRITLD, Shahid Beheshti University, M.C. Ahmad Reza Bahrmand Department of Mycobacteriology, Pasteur Institute of Iran Mohammad Reza Masjedi Department of Pulmonary Medicine, Ali Akbar Velayati Department of Pediatrics, NRITLD, Shahid Beheshti University, M.C., TEHRAN-IRAN. Journal Article 2021 01 01 Background: Prevention and treatment of drug-resistant clones is important in guiding TB control strategies. The simultaneous rapid detection of the type of mutation conferring resistance and the genotype reflect the extent of drug resistant TB transmission within the communities.Mutations conferring resistance to rifampin in rifampin-resistant clinical Mycobacterium tuberculosis isolates occur mostly in the 81 bp rifampin-resistance-determining region (RRDR) of the rpoB gene. Materials and Methods: Spoligotyping, IS6110- restriction fragment length polymorphism (RFLP) typing and sequencing of the rpoB gene were performed for 30 rifampin resistant M. tuberculosis isolates from patients referred to "Iranian National TB Laboratory" from 2006 to 2007. Results: Mutations in the RRDR of the rpoB gene were identified in 96.6% of rifampin-resistant isolates. The spoligotyping analysis identified one (3.3%) East African-Indian (EAI) family, 7 (23.3%) Haarlem family, 9 (30.0%) Beijing family and 12 (40.0%) Central Asia (CAS) family isolates. Sixty- six percent of CAS isolates carried a mutation in codon 516, 37% of Beijing isolates carried a mutation in codon 531 and 33% of Haarlem isolates carried a mutation in codon 526. Conclusion: Overall, there appeared to be a correlation between the genotype and specific mutations conferring resistance to rifampin in the Beijing and Haarlem families. (Tanaffos 2008; 7(2): 11-17) https://www.tanaffosjournal.ir/article_241256_b160ff415ef3d131127a5d4a9bbfe882.pdf