1
Department of Immunology, Iran University of Medical Sciences and Health Services,
2
Department of Infectious Disease, NRTILD, Shaheed Beheshti University of Medical Sciences and Health Services, TEHRAN-IRAN
Abstract
Background: Multi-drug resistant tuberculosis (MDR-TB), which is a worldwide clinical problem, is associated with high morbidity and mortality, as well as long-term survival of infected immunocompetent patients. In this study, the PPD-induced production of IL-12, IL-10, IFNγ, and IL-4 in peripheral blood mononuclear cells (PBMC) from patients with MDR-TB were investigated and compared with cytokine production capabilities in newly diagnosed, treated cases. Materials and Methods: This study investigated the profiles of IFN-γ, IL-12, IL-10, and IL-4 in response to a purified protein derivative (PPD) in peripheral blood mononuclear cells (PBMC) from 15 HIV negative patients with multidrug-resistant tuberculosis (MDR-TB), 11 newly diagnosed, treated cases and compared those with 10 healthy negative tuberculin reactors as controls. Results: ELISA results showed that the following stimulation with PPD, IFNγ production was significantly increased, whereas IL-10 was significantly reduced in MDR-TB patients compared with PPD negative controls. Production of IL-12 in MDR-TB patients showed elevation, induced by PPD stimulation of their PBMCs. However, MDR-TB patients were similar to healthy negative tuberculin controls in their IL-12 production and there was no statistically significant difference between them. IL-4 was detected to be in very low levels in three groups. Conclusion: In this study MDR-TB patients have no dysregulation in IL-12 or IL-10 production during Mycobacterium tuberculosis infection, and profiles are prone to Th1 cytokines. (Tanaffos 2004; 3(10): 25-31)
Shekar-Abi, M., Miandehi, N., Mansoori, S. D., Tavasoli Fayri, M., Alibahar, M., Amirkhani, A., & Mirsaeidi, S. M. (2004). The Study of Th1/Th2 Cytokine Profiles (IL-10, IL-12, IL-4, and IFNγ) in PBMCs of Patients with Multidrug Resistant Tuberculosis and Newly Diagnosed Drug Responsive Cases. TANAFFOS (Respiration), 3(2(spring)), 25-31.
MLA
Mehdi Shekar-Abi; Narges Miandehi; Seyed Davood Mansoori; Masoumeh Tavasoli Fayri; Mohammad Alibahar; Aref Amirkhani; Seyed Mehdi Mirsaeidi. "The Study of Th1/Th2 Cytokine Profiles (IL-10, IL-12, IL-4, and IFNγ) in PBMCs of Patients with Multidrug Resistant Tuberculosis and Newly Diagnosed Drug Responsive Cases". TANAFFOS (Respiration), 3, 2(spring), 2004, 25-31.
HARVARD
Shekar-Abi, M., Miandehi, N., Mansoori, S. D., Tavasoli Fayri, M., Alibahar, M., Amirkhani, A., Mirsaeidi, S. M. (2004). 'The Study of Th1/Th2 Cytokine Profiles (IL-10, IL-12, IL-4, and IFNγ) in PBMCs of Patients with Multidrug Resistant Tuberculosis and Newly Diagnosed Drug Responsive Cases', TANAFFOS (Respiration), 3(2(spring)), pp. 25-31.
VANCOUVER
Shekar-Abi, M., Miandehi, N., Mansoori, S. D., Tavasoli Fayri, M., Alibahar, M., Amirkhani, A., Mirsaeidi, S. M. The Study of Th1/Th2 Cytokine Profiles (IL-10, IL-12, IL-4, and IFNγ) in PBMCs of Patients with Multidrug Resistant Tuberculosis and Newly Diagnosed Drug Responsive Cases. TANAFFOS (Respiration), 2004; 3(2(spring)): 25-31.
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