TY - JOUR ID - 248017 TI - Gene Expression and In Vitro Pharmacogenetic Studies of Dopamine and Serotonin Gene Receptors in Tuberculosis JO - TANAFFOS (Respiration) JA - RSPR LA - en SN - 1735-0344 AU - Sheikhpour, Mojgan AU - Shokrgozar, Mohammad Ali AU - Biglari, Alireza AU - Pornour, Majid AU - Abdolrahimi, Farid AU - Poorazar Dizaji, Shahin AU - Khanipour, Sharareh AU - Masoumi, Morteza AU - Ebrahimzadeh, Nayereh AU - Abolfathi, Hanieh AD - Department of Mycobacteriology and Pulmonary Research, Microbiology Research Center, Pasteur Institute of Iran, Tehran, Iran AD - Department of Medical Genetics and Molecular Medicine, Faculty of Medicine, Zanjan University, Zanjan, Iran AD - National Cell Bank, Pasteur Institute of Iran, Tehran, Iran AD - Photo Healing and Regeneration Research Group, Medical Laser Research Center, ACECR, Tehran, Iran Y1 - 2021 PY - 2021 VL - 20 IS - 2 SP - 126 EP - 133 KW - Dopamine receptors KW - Serotonin receptors, Mycobacterium tuberculosis KW - Cytokines KW - autophagy DO - N2 - Background: Dopamine and serotonin receptors are present in lymphocytes, macrophages, and neutrophils, and have a mediating role in the immune system to respond to infections, including bacterial tuberculosis.Materials and Methods: In this study, at first, the changes in the expression pattern of 5 dopamine and 2 serotonin (5HTR2B & 5HTR2C) gene receptors were examined in the two groups of healthy and Tuberculosis patients using Real-Time PCR. Then pharmacogenetic studies aimed to induce autophagy on a lung monocyte cell line (THP1) infected with the standard strain of Mycobacterium tuberculosis (H37RV) were performed. Stimulation of the pro-inflammatory pathway by secreting cytokines before and after drug efficacy was investigated.Results: According to the result, dopamine receptor 2 genes showed decreased expression in patients with tuberculosis compared to normal individuals, and serotonin receptor genes showed increased expression. Additionally, with the effects of Bromocriptine and Fluoxetine, pro-inflammatory pathways were activated in macrophages infected with H37RV, and ELISA results showed that the levels of IL6 and TNFα secreted in these cells were significantly increased.Conclusion: According to the results, these receptors agonists or antagonists can activate the autophagy pathway to kill TB bacteria. UR - https://www.tanaffosjournal.ir/article_248017.html L1 - https://www.tanaffosjournal.ir/article_248017_bc57c91ca8829e2ff840483100e602bf.pdf ER -