TY - JOUR ID - 241479 TI - Detection of Pulmonary Tuberculosis by PCR Assay JO - TANAFFOS (Respiration) JA - RSPR LA - en SN - 1735-0344 AU - Sheikholslami, Maryam Fatemeh AU - Ziaee, Abd Ali AU - Khoshreza, Maryam AU - Masjedi, Mohammad Reza AU - Mohammadi, Foroozan AU - Farnia, Parisa AU - Velayati, Ali Akbar AD - Department of Clinical Anatomical Pathology, NRITLD, Shaheed Beheshti University of Medical Sciences and Health Services, AD - Department of Microbiology, Tehran University of Medical Sciences and Health Services AD - Department of Pulmonary Medicine AD - Department of Mycobacteriology AD - Department of Pediatrics, NRITLD, Shaheed Beheshti University of Medical Sciences and Health Services, TEHRAN-IRAN. Y1 - 2005 PY - 2005 VL - 4 IS - 1(winter) SP - 63 EP - 70 KW - tuberculosis KW - Pulmonary TB KW - PCR KW - Diagnosis DO - N2 - Background: Tuberculosis is a major world health problem mainly in the developing countries. Early isolation of infected patients and application of chemotherapy are the main prevention strategies for tuberculosis control. Although acid-fast stained smears and culture of M.tuberculosis are the standard procedures of diagnosis, they are low in sensitivity and time consuming, respectively. Polymerase chain reaction (PCR) technique has simplified and boosted the direct detection of M.tuberculosis in a significantly shorter time than two conventional methods mentioned. Materials and Methods: In this study, which was conducted in 9 months, 211 clinical samples were collected from patients with suspected M.tuberculosis complex who had referred to NRITLD. They were tested by PCR assay, culture technique and Ziehl-Neelsen staining. Two pair oligonucleotides were used in PCR assay, detecting 245bp of IS6110 sequence. Results of PCR assay were compared with those of culture technique. Results: One-hundred and forty five samples (68.7%) were sputum and the other 66 samples (31.3%) were bronchoscopy lavage. Twenty-five samples (11.8%) were positive by PCR assay out of which 21 (84%) were culture positive too.176 from 186 samples with PCR negative results were culture negative too. These 10 samples were examined for PCR inhibitor and identification of M.tuberculosis. Twenty percent (2 samples) of these had PCR inhibitor and 20% (2 samples) were not M.tuberculosis complex. Based on this study, after excluding the samples which had PCR inhibitor and non-tuberculosis complex, sensitivity and specificity of PCR assay in comparison with results of culture and Ziehl-Neelsen staining (as a gold standard) were 93.3% and 98.3%, respectively. Positive and negative predictive values were 84% and 94.6%, respectively. Conclusion: This study showed that there are no significant differences between cultures and PCR methods. For this reason, we can use PCR assay for direct detection of DNA from M.tuberculosis in clinical samples.(Tanaffos 2005; 4(13): 63-70) UR - https://www.tanaffosjournal.ir/article_241479.html L1 - https://www.tanaffosjournal.ir/article_241479_b056af30c8fc71087d36dc24a14c8e30.pdf ER -